Interrogating Glioma-Associated Microglia and Macrophage Dynamics Under CSF-1R Therapy with Multitracer In Vivo PET/MRI

ABSTRACT

Purpose

Glioma-associated microglia and macrophages (GAMMs) are key players in creating an immunosuppressive microenvironment. They can be efficiently targeted by inhibiting the colony-stimulating factor 1 receptor (CSF-1R). We applied noninvasive PET/CT and PET/MRI using 18F-fluoroethyltyrosine (18F-FET) (amino acid metabolism) and N,N-diethyl-2-[4-(2-18F-fluoroethoxy)phenyl]-5,7-dimethylpyrazolo [1,5-a]pyrimidine-3-acetamide (18F-DPA-714) (translocator protein) to understand the role of GAMMs in glioma initiation, monitor in vivo therapy-induced GAMM depletion, and observe GAMM repopulation after drug withdrawal.

Methods

C57BL/6 mice (n 5 44) orthotopically implanted with syngeneic mouse GL261 glioma cells were treated with different regimens using the CSF-1R inhibitor PLX5622 (6-fluoro-N-((5-fluoro-2-methoxypyridin-3-yl)methyl)-5-((5-methyl-1H-pyrrolo[2,3-b]pyridin-3-yl)methyl)pyridin-2-amine) or vehicle, establishing a preconditioning model and a repopulation model, respectively. The mice underwent longitudinal PET/CT and PET/MRI.

Results

The preconditioning model indicated similar tumor growth based on MRI (44.5%6 24.8%), 18F-FET PET (18.3%6 11.3%), and 18F-DPA-714 PET (16% 6 19.04%) volume dynamics in all groups, suggesting that GAMMs are not involved in glioma initiation. The repopulation model showed significantly reduced 18F-DPA-714 uptake (245.6% 618.4%), significantly reduced GAMM infiltration even after repopulation, and a significantly decreased tumor volume (254.29% 6 8.6%)with repopulation as measured by MRI, supported by a significant reduction in 18F-FET uptake (250.2% 6 5.3%).

Conclusion

Authors

Foray, Claudia;  Barca, Cristina;  Winkeler, Alexandra; Wagner, Stefan; Hermann, Sven; Schäfers, Michael; Grauer, Oliver Martin;  Zinnhardt, Bastian; Jacobs, Andreas H.​